Lymph nodes were visualized in B-NDG Tg(RORγt-Il2rg) mice dyed by Evans blue. B-NDG mice (n=6) and B-NDG Tg(RORγt-Il2rg) mice (n=10) were sacrificed and intravenously injected with 10% Evens blue in the planta pedis. The red arrows indicate the lymph nodes in inguinal, axillary and mesenteric site of B-NDG Tg(RORγt-Il2rg) mice. No lymph node was visualized in B-NDG mice.